Abstract
Abstract Serum hormones and metabolites of lean and obese gilts were determined. Fifteen Iberian and fifteen Landrace gilts, of approximately 20 kg body weight, were fed isoenergetic (13.6 kJ ME/g DM, semisynthetic diets either with equilibrated amino acid profile at two crude protein (CP) levels (12% (0.81% lysine (Lys)) and 16% CP (1.08% Lys) as-fed basis) or lysine deficient (12% (0.30% Lys) or 16% CP (0.36% Lys) as-fed basis for Iberian and Landrace, respectively). Lysine deficient diets were offered only at the optimal protein level for maximum protein accretion for each breed. Each dietary treatment was assayed in 5 animals. Gilts were allocated in metabolic cages at 21 ± 1.5 °C with free access to water and fed four times daily at 90% ad libitum during 10 days. On day 11th blood samples were taken 5–5.5 h postprandial and serum obtained and frozen at − 20 °C. Growth hormone (GH), insulin like growth factor I (IGF-1), insulin, leptin, glucose, urea, creatinine, cholesterol and triglycerides were determined. Landrace gilts had higher serum glucose (P These data suggest that Iberian and Landrace growing gilts have distinct hormone and metabolite serum profiles which are altered by a severe dietary lysine deficiency.
Published Version
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