Abstract
OBJECTIVE: To determine whether serum osteocalcin levels can be used as a marker for assessing skeletal maturity. MATERIALS AND METHODS: The sample consisted of 60 patients aged 08-20 years (30 males, 30 females) reporting to Department of Orthodontics and dentofacial orthopedics, and Department of Pedodontics ,Faculty of dental sciences , Ramaiah university of Applied sciences , Bangalore. Lateral cephalometric radiographs were obtained from all the subjects. Cervical staging of orthodontic patients satisfying the inclusion criteria were evaluated on their respective lateral cephalograms by using the Hassel and Farman method. After cervical vertebral maturation evaluation, 60 subjects selected for the study from the screened patients were grouped into 6 cervical stage (CS) groups of 10 per group (5 males, 5 females). 1ml blood sample was collected from each patient after taking informed consent. The collected blood was centrifuged for 20 min at room temperature and then stored at -20°C until analysis. The serum samples were subjected to ELISA test for determining levels of Osteocalcin.
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