Abstract
MicroRNAs (miRs) have been shown to be differentially expressed in the serum of cancer patients and controls, and can thus be used as biomarkers for cancer screening. We detected the expression level of miR-155 in the serum of female breast cancer patients and healthy controls to investigate whether serum miR-155 could discriminate patients with early-stage breast cancer. Serum samples were collected from 20 female patients with newly diagnosed breast cancer and 10 healthy controls. Real-time quantitative PCR was used to detect the expression level of miR-155. The expression level of miR-155 was significantly increased in the serum of breast cancer patients compared with in the serum of normal controls. MiR-155 may be useful as a blood-based biomarker for breast cancer screening.
Highlights
MicroRNAs have been shown to be differentially expressed in the serum of cancer patients and controls, and can be used as biomarkers for cancer screening
The cycle threshold (Ct) value is the number of quantitative PCR (qPCR) cycles required for the fluorescent signal to cross a specified threshold. ∆Ct was calculated by subtracting the Ct values of RNU6B from those of miR-155. ∆∆Ct was calculated by subtracting the ∆Ct of the control samples from the ∆Ct of the cancer samples
This report describes a quantitative assessment of serum miR-155 levels in female breast cancer patients
Summary
Zhao S Y, et al Chin Sci Bull September (2012) Vol. No.26 study. Blood samples were collected from 30 women including 20 breast cancer patients and 10 healthy subjects as normal controls, aged between 35 and 65 years old. Zhao S Y, et al Chin Sci Bull September (2012) Vol.. Blood samples were collected from 30 women including 20 breast cancer patients and 10 healthy subjects as normal controls, aged between 35 and 65 years old. Blood was collected from the antecubital fossa and placed in a serum separator tube. The collected blood was allowed to stand for no more than 1 h at room temperature before being centrifuged at 2465 × g for 5 min to obtain serum [1]. The resultant serum was aliquoted into Eppendorf tubes and stored at –80°C
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