Abstract
Circulating microRNAs (miRNAs) have been reported dysregulated during exercise. However, the changes of specific serum miRNAs during the 5-km run test with intensity of 51–52% maximum oxygen uptake (V̇O2max) and their association with traditional cardiovascular-related indicators remain well-characterized. Levels of miR-1, miR-21, miR-146a, miR-155, miR-181, and miR-210 were detected in 120 young subjects before and after the exercise training by quantitative reverse-transcription PCR (RT-qPCR). Besides, the levels of cardiac troponin I (cTNI), myoglobin (Myo), creatine kinase (CK), creatine kinase-MB (CK-MB), aspartate aminotransferase (AST), lactate dehydrogenase (LDH), ischemia-modified albumin (IMA), interleukin-6 (IL-6), and C-reactive protein (CRP) were measured and the correlation between levels of serum miRNAs and biochemical parameters was also analyzed. Compared with resting state, the serum levels of miR-1, miR-146a, miR-155, miR-181, and miR-210 were significantly increased after exercise training. Serum levels of miR-146a, miR-155, and miR-210 after exercise training were positively correlated with Myo, CK-MB, and LDH, respectively, while miR-1, miR-146a, miR-181, and miR-155 were positively correlated with the levels of IL-6. Additionally, all the five miRNAs were negatively correlated with IMA levels. The multivariate logistic regression analysis showed that high levels of miR-146a, AST, LDH, and IL-6 in serum were risk factors, while low IMA contents were a protective factor for cardiovascular adaptation during exercise. In conclusion, the dynamic changes of miRNAs under the condition of the 5-km continuous running contribute to the adaptive regulation of the cardiovascular function of the body.
Highlights
The 5-km run test is the basic training item and the main content of endurance training for subjects
We examined the clinical biochemical parameters that are associated with cardiovascular disease (CVD) and compared their changes before and after training
We found that serum Myo, creatine kinase (CK)-MB, AST, lactate dehydrogenase (LDH), and IL-6 were significantly increased, while the levels of ischemia-modified albumin (IMA) were significantly decreased after the 5-km running training, indicating that a distinct exercise adaptation in young healthy subjects
Summary
The 5-km run test is the basic training item and the main content of endurance training for subjects. Numerous studies demonstrated that miRNAs are novel indicators for cardiovascular disease (CVD) risk prediction, condition monitoring, and prognosis, which can regulate heart development and physiological function (Ultimo et al, 2018). MiRNAs have a higher diagnostic value for CVD when compared with the established gold standard (de GonzaloCalvo et al, 2017). It reports that exercise alters the miRNAs profile and these altered miRNAs have been observed to participate in metabolic pathways related to myocardial function, metabolism, and inflammatory response (Schuler et al, 2013). No robust correlation was identified between changes of these miRNAs and myocardial marker, inflammation indicator, or ischemia marker, indicating that further studies are still required to identify the potential use of the circulating miRNAs of exercise
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