Abstract
Purpose: To investigate the precise function of miRNA-126-3p in controlling the expression of the VEGF gene and the signalling cascade that it is linked to in patients with type 2 diabetes Mellitus (DM). Study Design: Cross-sectional analytical study. Place and Duration of Study: Prof Ngoerah General Hospital as a tertiary hospital in Bali, Indonesia from January-April 2022 Methods: This study involved 35 vision-threatening diabetic retinopathy (VTDR) and 30 non-VTDR patients of type 2 DM. After diagnosis of DR by vitreoretinal specialist, blood samples were drawn. Real-time polymerase chain reaction assays were performed to examine miRNA-126-3p and ELISA to detect VEGF. Continuous data were analyzed using median (minimum-maximum range) and categorical data were analyzed using percentages. The statistical analysis was conducted using SPSS for Windows version 25.0.Thedifference and relationship between the proportions were tested by the Chi-square test and logistic regression test. Results: In case group, there were 23 subjects (65.7%) with low serum miRNA-126-3p expression, while in the control group, there were 7 subjects (23.3%) with low serum miRNA-126-3p expression (OR=6.3; 95% CI 2.1-18.86). In the case group, 20 individuals (57.1%) had high serum VEGF levels of 71.6 ng/L, whereas in the control group, there were 6 individuals (20.0%) with high serum VEGF levels. There was a statistically significant difference between the two groups (OR=5.33; 95% CI 1.75-16.3). Conclusion: The miRNA-126-3p expression and serum VEGF levels are the risk factors for VTDR development in patients with type 2 diabetes.
Published Version
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