Serum level of microRNAs and their associations with diabetic kidney disease in Uygur patients

Abstract

Objective To investigate the expression and clinical significance of serum microRNA(miRNA) in Uygur patients with diabetic kidney disease(DKD). Methods A total of 50 Uygur patients with type 2 diabetes mellitus(T2DM) were collected from March 2015 to December 2015 in the Department of Endocrinology and Physical Examination Center of the First Affiliated Hospital, Xinjiang Medical University. Patients were divided into 2 groups: T2DM group[24 h microalbunminuria(MAU)<30 mg/24 h, n=25]and DKD group (MAU≥30 mg/24 h, n=25). Another 25 Uygur healthy volunteers were included as control group(n=25).The level of serum miRNAs were measured by real-time polymerase chain reaction, and the associations between miRNAs with clinical parameters in diabetic nephropathy were analyzed. Results The expression of miR-21, miR-187, miR-451 were decreased in T2DM group(0.7±0.4, 0.8±0.4, 0.7±0.4) and DKD group(0.7±0.4, 0.8±0.3, 0.8±0.6)compared with those in control group(1.5±0.9, 1.2±0.7, 1.7±1.0, t=2.29-3.99, all P<0.05). The miR-145 level was significantly decreased in DKD group compared with that in T2DM group or control group (0.6±0.3 vs 0.9±0.4, 0.6±0.3 vs 1.1±0.6, t=2.50, 3.06, all P<0.05) . The level of serum miR-21, miR-187, miR-145, or miR-451 was positively correlated with eGFR (r=0.365-0.743) , but inversely correlated with SCr and UAER (r=-0.545--0.223,all P<0.05) . The level of serum adiponectin was decreased in T2DM group and DKD group compared with that in control group. However, the adiponectin level was higher in DKD group than in T2DM group (t=-2.11-4.18, all P<0.05). miR-21, miR-187, miR-145, and miR-451 were significantly correlated with adiponectin level (r=0.281-0.671, all P<0.05) . Conclusion miR-21, miR-187, miR-145 and miR-451 may be involved in the pathogenesis of DKD, and may be potential diagnostic biomarkers of DKD. Key words: Diabetic kidney disease; MicroRNA; Adiponectin; Glomerular filtration rate