Abstract
Aim. To determine the role of organic acids in the pathogenesis of missing abortion.Materials and Methods. To analyze the endometrial organic acid content, we applied high performance liquid chromatography. Sampling was performed from 20 women with missed abortion and 10 patients with normal pregnancy utilising the vacuum aspiration. Following protein separation by means of double centrifugation and ultrafiltration, supernatant was profiled for the organic acids.Results. Women who experienced missing abortion had significantly higher concentrations of serum and endometrial lactic acid concentrations (10.01 ± 0.16 and 8.28 ± 0.20 mmol/L, respectively) than those with healthy pregnancy (8.16 ± 0.24 and 7.57 ± 0.20 mmol/L, respectively). In contrast, endometrial pyruvic acid level was lower in abortion group (27.71 ± 0.04 mmol/L) as compared to the controls (34.1 ± 0.40 mmol/L). Furthermore, 4-hydroxyphenylacetic acid was found exclusively in women with missed abortion.Conclusion. Changes in organic acid profile may play a major role in pathogenesis of missed abortion. Serum and endometrial 4-hydroxyphenylacetic acid can be suggested as a novel biomarker of early pregnancy loss.
Highlights
Following protein separation by means of double centrifugation and ultrafiltration, supernatant was profiled for the organic acids
Changes in organic acid profile may play a major role in pathogenesis of missed abortion
Serum and endometrial 4-hydroxyphenylacetic acid can be suggested as a novel biomarker of early pregnancy loss
Summary
Определить роль органических кислот в патогенезе неразвивающейся беременности. Были обследованы 2 группы пациенток (всего 30 женщин): основную группу составили 20 женщин с неразвивающейся беременностью (НБ), группу сравнения – 10 женщин с физиологически протекающей беременностью. Количественную оценку органических кислот проводили, сравнивая результаты анализа проб с внешними стандартами при 214 нм. У женщин основной группы в сыворотке крови и эндометрии концентрация молочной кислоты составила 10,01± 0,16 и 8,28± 0,20 ммоль/л соответственно, что было достоверно выше, чем у женщин в группе сравнения (8,16 ± 0.24 and 7,57 ± 0,20 ммоль/л). В основной группе в эндометрии были снижены концентрации пировиноградной кислоты более чем на 10 ммоль/л (27,71 ± 0.04 ммоль/л), что также достоверно отличалось от группы сравнения (34,1 ± 0.40 ммоль/л). Профиль органических кислот у женщин с неразвивающейся беременностью // Фундаментальная и клиническая медицина.
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