Abstract
Cross-sectional study, which combined multistage cluster sampling and simple random sampling, was employed with the objectives of investigating seroprevalence, associated risk factors, molecular detection and isolation of Peste Des Petits Ruminants Virus (PPRV) in sheep from October 2019 to August 2019 in selected districts of Horo Guduru Wollega Zone. A total of 387 serum samples were collected from 58 flocks comprised of 387 sheep population. Competitive Enzyme-Linked Immunosorbent Assay (cELISA) was used to detect the presence of PPRV specific antibodies in the sera of animals. Pearson’s Chi-Square and logistic regression analyses were used to see the association of PPR seroprevalence with potential risk factors. The flock-level overall seroprevalence of PPR was found to be 25.86%. An overall seroprevalence of 6.98% (95% CI: 4.65, 10.00) was recorded in the study areas. The seroprevalence of PPR in sheep was significantly higher in mid-highland than highland and lowland (P=0.029). Though the overall seroprevalence of PPR in this study was low, the seropositivity of sheep was due to natural infection indicating the PPRV infection has been circulating in the study areas. Therefore, regular vaccination should be given for sheep to control and prevent its further distribution and awareness should be created for farmers on identified potential risk factors.
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