Abstract
Toxoplasma gondii RH tachyzoites replicated in VERO cell cultures. A rosette of many tachyzoites is shown at left and several parasite pairs at right (arrows) Results: In both cell types invasion with either strain increased along time; however, proportion of infected cells was lower for HUVECs than for HMEC-1. Also, the strains differed in invasion kinetics: ME49 parasites were faster than RH ones, regardless of cell type. Finally, both HMEC-1 and HUVECs showed higher numberofparasitic vacuolesper cellwhen infectedbyME49 tazhyzoites than by RH protozoan, i.e ≈30 vs ≈20 at 4 hours, respectively. Conclusion:Results suggest thatHMEC-1 cells aremore susceptible to infection by T. gondii than HUVECs. This might be related to cell cycle-progress -which is usually badly regulated in cell linesand T. gondii is more invasive during the G1-S phase. The unexpected observation that RH parasites are slower than ME49 ones might be related to their higher ability to survive out of the cell.
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