Abstract
Serological and Molecular Comparison Study for Diagnosis of Cytomegalovirus Infection in aborted Pregnant Women in Iraq
Highlights
Viral infections in pregnancy are the major causes of maternal and fetal morbidity and mortality
The clinical manifestations of neonatal infections vary depending on the viral agent and gestational age at exposure
The species that infect human is commonly known as human cytomegalovirus (HCMV), Infections HCMV may be shed in bodily fluid in human intermittently with no detectable signs and symptoms of the infection (Abbas et al, 2017; Tyagi, 2012)
Summary
Viral infections in pregnancy are the major causes of maternal and fetal morbidity and mortality. Quantitation of the viral nucleic acid by amplifying the target sequence thousands-fold In most cases, they are considered a reference, or ‘gold standard’ method for diagnostic practices such as screening donated blood for transfusion-transmitted viruses like CMV, HIV, HCV (Jackson, 1990). The most widely used variants of conventional amplification are real-time PCR (quantitative PCR) and reverse transcription-PCR (RT-PCR) Both are nowadays becoming benchmarks in assessing the viral load, and while the first method quantifies DNA throughout the reactions in real time (Ntziora et al, 2013), the second performs RT of the mRNA and amplifies the resulting cDNA (complementary DNA), it quantifies RNA. The main advantages of these methods are the decrease of contamination and the possibility for quantitative applications (Kwoh et al.,1989)
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