Abstract
Herpes Simplex Viruses (HSV) and Human Cytomegaloviruses (HCMV) are the major cause of serious viral complications in pregnant women. Screening methods that is ELISA for detecting Herpes Simplex virus(HSV) and Human Cytomegalovirus(HCMV) tend to be slow and insensitive. Therefore in this work, a rapid Real Time PCR (qT-PCR) and ELISA based assay was designed to detect HSV and CMV among pregnant women in Iraq from serum samples. Our study revealed that among the ELISA screened 420 sera samples, 81 (19.3%) of CMV and 17(4.05 %) of HSV were positive, while 64 (12.9%) had positive results for CMV DNA and 5(1.2%) had HSV DNA through real-time PCR. Real time PCR was more sensitive and reliable method in diagnosis of CMV and HSV infections in pregnant women in this comparative study.
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