Abstract
WU polyomavirus (WUPyV) and KI polyomavirus (KIPyV) are novel human polyomaviruses. They were originally identified in human respiratory secretions, but the extent of human infection caused by these viruses has not been described to date. To determine the seroepidemiology of WUPyV and KIpyIV, we used an ELISA to screen serum samples from 419 patients at the St. Louis Children's Hospital and Barnes-Jewish Hospital during 2007-2008. The age-stratified deidentified samples were examined for antibodies to the major capsid proteins of WUPyV and KIPyV. Seropositivity for each virus was similar; antibody levels were high in the youngest age group (<6 months), decreased to a nadir in the next age group (6 to <12 months), and then steadily increased with subsequent age groups, eventually reaching a plateau of approximate, equals 80% for WUPyV and approximate, equals 70% for KIPyV. These results demonstrate that both KIPyV and WUPyV cause widespread infection in the human population.
Highlights
WU polyomavirus (WUPyV) and KI polyomavirus (KIPyV) are novel human polyomaviruses
Previous studies on BK virus (BKV), JC virus (JCV), and simian virus 40 (SV40) polyomaviruses indicated that the viral major capsid protein is immunodominant and that human serum can recognize the recombinant viral protein 1 (VP1) expressed in bacteria [16]
We demonstrate that the full-length recombinant WUPyV VP1 and KIPyV VP1 expressed in bacteria are capable of detecting antibodies against WUPyV and KIPyV VP1, respectively, using either the ELISA or the Western blot assay format
Summary
WU polyomavirus (WUPyV) and KI polyomavirus (KIPyV) are novel human polyomaviruses They were originally identified in human respiratory secretions, but the extent of human infection caused by these viruses has not been described to date. Seropositivity for each virus was similar; antibody levels were high in the youngest age group (
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