Sericin-reinforced dual-crosslinked hydrogel for cartilage defect repair

Abstract

Articular cartilage is essential for normal daily joint function activities. However, it is difficult for articular cartilage to repair itself after injury due to the lack of nerves and blood vessels, so an effective cartilage repair method is necessary. As a three-dimensional polymer network structure with high water content, hydrogel is a good candidate material for cartilage repair, and it is also a research hotspot in the treatment of cartilage injury. Here, a porous dual-crosslinked hydrogel containing sodium alginate (SA) and silk sericin (SS) was designed for in situ repair of cartilage damage. The degradation rate of the hydrogel was regulated by changing the content of SS to match the rate of cartilage regeneration. The hydrogel had excellent mechanical properties (compressive strength≈245 kPa, compressibility≈60%), high water content (85%−88%) and porosity(>20%), and when the content of SS is 1%, the scaffold has the best comprehensive performance. Existing excellent cytocompatibility, the scaffold can promote the adhesion and proliferation of chondrocytes while reducing inflammatory cell infiltration. The cartilage defect repair experiments in vivo showed that artificial cartilage was formed at 4 weeks with molecular structure similar to natural cartilage. It is expected to be applied to clinical cartilage repair through the dual-crosslinked three-dimensional cartilage scaffold.