Abstract
Objective To observe the efficacy of transfection of transforming growth factor-β1 (TGF-β1) gene into rabbit bone marrow stromal cells (MSCs) mixed with calcium alginate to repair defects of articular cartilage of the rabbit knee.Methods The bone marrow was obtained from a four-weekold New Zealand rabbit,and the bone marrow stromal cells were isolated and purified in vitro.Twenty-four adult New Zealand rabbits were chosen and divide into 4 groups randomly.Full-thickness articular cartilage defects were made in the non-weight-boating area of the bilateral femoral condyles in New Zealand rabbits.The different 4 groups were transferred with complex component to the different defect place of the articular cartilage:group A,MSCs + calciumalginate complex,which has been transfected by TGF-β1 (experimental group) ; group B,MSCs + calciumalginate complex,which has been transfected by empty plasmid (control group Ⅰ) ; group C,calciumalginate without cells (control group Ⅱ) ; group D,without any treatment (control group Ⅲ).The femoral condyles were harvested at the end of 12th week,and the repair of cartilage defects observed and changes in histology and immunohistochemistry were assessed.The repairing effect was evaluated by using O' Driscoll' s.Results Repaired tissue in experimental group was approximately smooth,consisting of a large amount of type-Ⅱ collagen.In 3 control groups,the type-Ⅱ collagen in the repaired tissue was bit and had apparent defect.At the 12th week,the histomorphology scores in experimental group and 3 control groups,O' Driscoll,Keeley and Salter,were respectively (17.000 ±1.758) for experimental group,(7.830 ± 1.528) for control group Ⅰ,(6.580 ± 1.379) for control group Ⅱ and (3.170 ± 1.267) for control group Ⅲ.The comparison and analysis of single factor varience between groups indicated that there was statistically significant difference when experimental group and 3 control groups were compared inpairs.Conclusion The group of implanted TGF-β1 + MSCs is superior to control groups in the effect of repairing cartilage defects. Key words: Cartilage repair; Transforming growth factor-β1; Marrow stromal cells; Gene transfection
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