Abstract
In this contribution, a channel aspect ratio of >2 was used to access high velocity regimes to provide confined sample cores by Dean focussing in advance of linear inertial focussing. This produces a singular separation origin with a mirrored transport path for efficient particle and blood cell sorting, while also increasing the spatial resolution for multiscale sorting.
Highlights
A channel aspect ratio of >2 was used to access high velocity regimes to provide confined sample cores by Dean focussing in advance of linear inertial focussing
We provide a generalised framework for the straightforward design of inertial microfluidic fractionation systems for resolving cellular and sub-cellular samples
Summary
Increasing mean flow velocity and associated shear allows the effect of the deformation lift force to be observed when processing blood cells to provide an additional fractionation dimension. Increased velocity enables the integration of upstream Dean focussing of the sample into a confined core stream. This approach collapses inertial transport into a singular horizontal path, eliminating initial positional dissimilarities to increase the efficiency of size-based digital binning. This emphasises the performance gains when moving from sheathless to 2D and 3D focussing. We provide experimentally derived quantitative design rules that can be extended to other microparticle and cell sorting scenarios
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
