Abstract
Serial block-face scanning electron microscopy (SBF-SEM) can be used to image large (100-micrometer size) volumes of biological tissue, and thus complements standard transmission electron tomography, as well as scanning transmission electron tomography. SBF-SEM is attractive not only for its ability to generate high-resolution three-dimensional ultrastructure, but also for its relative ease of implementation. Whereas electron tomography traditionally requires the tissue block to be manually sectioned, SBF-SEM makes use of an automated ultramicrotome built in to the SEM's specimen chamber; and tissues optimally stained with heavy metals and imaged with backscattered electrons, show equivalent contrast to TEM images of conventionally stained sections (Denk and Horstmann, 2004).
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