Abstract
In this contribution, the utility of sequential injection analysis in combination with surface-enhanced Raman spectroscopy (SERS) as a detection technique was investigated for simultaneous determination of aspirin and vitamin C in their pharmaceutical dosage forms and in spiked urine samples. The silver substrate was synthesized in situ by laser-induced photochemical procedure. By focusing the laser on a flow cell at 1ml/min of continuous flow of 0.5mM silver nitrate and 5mM sodium citrate mixture, an active silver spot on the inner wall of the flow cell was prepared in a few seconds. The whole setup is fully computer controlled using ATLAS software to combine the two techniques. The system allows sequential determination of aspirin concentrations ranging from 100 to 500ng/ml and vitamin C concentrations between 10 and 110ng/ml with good precision of relative standard deviations (RSDs) of 0.85 and 1.7%, respectively. A comparison of these results with those of the reported procedures showed excellent results compared with t and F values, indicating good accuracy and precision. The detection limits were 32 and 3ng/ml for aspirin and vitamin C, respectively.
Published Version
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call