Sequential One‐Pot Three‐Enzyme Synthesis of the Tetrasaccharide Repeating Unit of Group B Streptococcus Serotype VIII Capsular Polysaccharide

Abstract

Comprehensive SummaryThe biochemical property and functional identification of three recombinant glycosyltransferases, including β‐1,4‐rhamnosyltransferase (Cps8R), β‐1,4‐galactosyltransferase (Cps8J) and α‐2,3‐sialyltransferase (Cps8K) involved in the biosynthesis of the tetrasaccharide repeating unit of serotype VIII capsular polysaccharide (CPS) of Group B Streptococci (GBS), were systematically investigated. Subsequently, these recombinant enzymes were employed for one‐pot three‐enzyme efficient synthesis of the tetrasaccharide repeating unit of GBS serotype VIII CPS using the chemically synthesized Glcα‐PP‐(CH2)11‐OPh as the starting substrate in a satisfying yield of 87%.