Abstract
Enantioanalysis of biomedical substances is very important for clinical analysis because the L- and D-enantiomers of the same chiral compound may be markers for different illnesses. A sequential injection analysis (SIA) system using amperometric biosensors as detectors is proposed for the simultaneous determination of L- and D-pipecolic acid (PA). The amperometric biosensors were based on L-amino acid oxidase (L-AAOD) or/and horseradish peroxidase (HRP) for the assay of L-PA and D-amino acid oxidase (D-AAOD) and/or HRP for the assay of D-PA. The linear concentration ranges are of pmol/L to nmol/L magnitude order, with very low limits of detection. The SIA/biosensors system can be used reliably on-line for the simultaneous assay of L- and D-PA from serum samples, with a frequency of 34 samples per hour.
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