Abstract
Tea polyphenols (TP) and tea polysaccharides (TPS) are the two main hypoglycemic substances in tea. While the hypoglycemic activities of TPS and TP have been extensively reported, there remains a paucity of evidence regarding their synergistic regulation of hypoglycemic function. In this study, water-soluble polysaccharides and cell wall polysaccharides were firstly prepared from Yichang roasted green tea using sequential extraction method. Different types of tea polysaccharides, especially tea cell wall polysaccharides, exhibited diverse chemical compositions, molecular structures, and biological activities. The tea cell wall polysaccharides extracted with 4M NaOH (p4M-TPS) exhibited the highest inhibitory activity against different glycosidases. Furthermore, TPS (p4M-TPS) and TP (Epigallocatechin gallate, EGCG) were combined in different proportions to assess their hypoglycemic effect on hyperglycemic mice, and p4M-TPS-TP1:2 exhibited the most pronounced effect in promoting the expression of the TGR5/GLP-1 pathway to exert the synergistic anti-diabetic potential. This study firstly purified and characterized tea cell wall polysaccharide, and explored its potential hypoglycemic activity, thereby providing a valuable reference for the development of natural hypoglycemic functional products originating from tea active ingredients and their combinations, indicating the synergistic effect of tea active ingredients rather than just a single active ingredient.
Published Version
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