Abstract
A novel approach that simplifies the laser source requirements for confocal and multiphoton laser scanning (CLSM and MPLSM) using the novel dispersion properties of photonic crystal fibre (PCF) is reported. By tuning the fs-pulsed Ti:Sapphire laser to the zero dispersion wavelength of the PCF, the spectral and temporal properties of the source are largely unaffected and hence this source can easily be used for MPLSM. Conversely, by tuning the Ti:Sapphire laser emission wavelength by less than 10 nm to anomalously pump the PCF, the resultant white-light supercontinuum source can perform CLSM. Sequential CLSM and MPLSM of a double-labelled guinea pig detrusor (smooth muscle layer) specimen is described.
Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
