Abstract
Acinetobacter baumannii is one of the opportunistic nurses responsible for many acquired infections in hospitals due to its ability to resist many antibiotics. This is one of the problems facing hospitals in the world. Identification of the cvaC gene and sequence analysis in Acinetobacter baumannii isolates from various infections, and mutation detection in this gene. From 1st of September to 30th of November, 2016, 200 Acinetobacter baumannii isolates were obtained from various clinical samples. Follow fifty isolates from blood, twenty isolates from urinary tract infections, thirty isolates from wound infections, forty isolates from burn infections and twenty-five isolates from stool samples from various hospitals (Central Children's Hospital, Al Karama Hospital, Karkh General Hospital, Al-Ameen Medical City Hospital, Educational Labs, Baghdad Teaching Hospital, Child Protection Hospital, Burns and Wounds Hospital) in Baghdad city. Identification forty isolates confirmed that they belong to Acinetobacter baumannii, including fourteen isolates from a stool sample, nine isolates from blood, eight isolates from burns, four isolates from wound infections, and Respiratory tract infection, , and only one isolate from urinary tract infections sample. Genotypic detection of the cvaC gene of Acinetobacter baumannii showed the presence of this gene in 16 isolates (40%) and Sequencing analysis of cvaC has shown seven genetic mutations and only one mutation has been converted amino acid Alanine to Valine.The amino acid Alanine was changed to Valine in Position 656 at Subject 678, resulting in silent mutations that did not affect protein translation and other mutations that resulted in a change in the amino acid arrangement and protein translation.
Highlights
Acinetobacter baumannii is a Gram-negative, non-motile, strict aerobic, non-fermented lactose, coccobcilli, catalase-positive, and oxidase-negative [1,2]
Identification forty isolates confirmed that they belong to Acinetobacter baumannii, including fourteen isolates from a stool sample, nine isolates from blood, eight isolates from burns, four isolates from wound infections, and Respiratory tract infection, and only one isolate from urinary tract infections sample
Genotypic detection of the cvaC gene of Acinetobacter baumannii showed the presence of this gene in 16 isolates (40%) and Sequencing analysis of cvaC has shown seven genetic mutations and only one mutation has been converted amino acid Alanine to Valine
Summary
Acinetobacter baumannii is a Gram-negative, non-motile, strict aerobic, non-fermented lactose, coccobcilli, catalase-positive, and oxidase-negative [1,2]. It is known for its capacity to build biological membranes [3,4] These opportunistic bacterial pathogens cause many opportunistic infections in hospitals, including pneumonia, burns infection, wound infection, septicemia, endocarditis, and urinary tract infection. A. baumannii is one of the opportunistic nurses responsible for many acquired infections in hospitals due to its ability to resist many antibiotics. This is one of the problems facing hospitals in the world and comes after Pseudomonas aeruginosa was isolated from hospital environments [8, 9, 10, 11]. Colicin V is a peptide antibiotic produced by members of the Enterobacteraceae family and A. baumannii that kills bacteria of other species. The solution of each initiator was present separately at 10 pmol/μl by adding 10μl from each stock solution to 90 μl of D.W. and mixed well with Vortex mixture, keeping with stock solutions at 20°C and mixing the initiator solution after removing it from ice using the Vortex carburetor prior to use
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