Abstract
Background: Forty isolates were obtained of Acinetobacter baumannii from (200) samples, the samples were collected from different cases including: - wounds, burns, Stool, Urinary tract infection urine.
 Aim of study: The aim of study is to isolate and diagnose Acinetobacter baumanniii from different clinical cases and to investigate the fimH gene.
 Objective: Respiratory tract infection and blood sample.For the period between 1/9/2016 to 30/11/2016.
 Materials and methods: Identification of 40 isolates confirmed to be Acinetobacter baumannii included 9 isolates from blood, 1 isolate from urinary tract infections, 4 isolates from each of wound infections and Respiratory tract infection, 8 isolates from burns and 14 isolates from stool sample.
 Results: The result revealed that the fimH gene was present in (20) isolates (50%) of Acinetobacter baumannii. The result Showed (7 (isolate (17.5%) of Stool and blood has fimH gene to each of them and Burns (3) isolates (7.5%) has fimH gene and (2) isolate (5%) of respiratory tract infection has fimH gene and finally only (1) isolate (2.5 %) of Wound infection. While all isolated for each of the urinary tract infections doesn't the fimH gene. The gel electrophoresis showed that the molecular weight of fimH gene was 508 bp. 
 Conclusion: Sequential analysis detection of fimH showed three silent mutations that did not affect the amino acid translation.
Highlights
These bacterial opportunistic pathogens are responsible for many opportunistic infections in the hospital including pneumonia, burns infection wound infection, septicemia, endocarditis and urinary tract infection [3, 4]
Detection of the fimH gene: Prepare the primer solution for FimH F Forward and FimH R Reveries accordance the instructions of the manufacturer Alpha (DNA) using sterilized ionic distilled water to obtain a concentration of 100 picommol / microliter and present each solution of the initiates separately at 10 picomol / microliter by taking 10 microliters of the Stock Solution and add 90 microliters of distilled ionic distilled water and store the stock solutions under - 20 ° C, mixing of the solution after removing it from the ice using vortex before use
The results showed that 19 isolates (47.5%) of A. baumannii possessed the fimH gene
Summary
Acinetobacter species are Gram negative bacteria , Strict aerobic, catalase positive, oxidase negative, non-motile, but some species bacteria have a kind of movement Twitching Motility because of polarity fimbriae [1, 2] These bacterial opportunistic pathogens are responsible for many opportunistic infections in the hospital including pneumonia, burns infection wound infection, septicemia, endocarditis and urinary tract infection [3, 4]. Acinetobacter species include several types of A. baumannii species These bacteria have many of the virulence factors that help them cause disease like capsule formation, production of biofilm and production of enzymes such as lipase, protease, Gelatinase activity, Pellicle assay, Siderphores, production of colic in, formation of polysaccharides, adhesion to living cells and non-living surfaces [7, 8]. Forty isolates were obtained of Acinetobacter baumannii from [200] samples, the samples were collected from different cases including: - wounds, burns, Stool, Urinary tract infection urine. Conclusion: Sequential analysis detection of fimH showed three silent mutations that did not affect the amino acid translation
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