Abstract
The actinomycete Frankia alni fixes N 2 in root nodules of several non-leguminous plants. It is one of the few known N 2-fixing members of the high-GC Gram + lineage of prokaryotes. Thus, we have undertaken a study of its nitrogen fixation gene ( n/f) organization to compare with that of the more extensively characterized proteobacteria. A cosmid (pFN1) containing the n/f region of Fa CpI1 was isolated from a cosmid library using the nifHDK genes of Fa CpI1 as a probe. A 4.5-kb BamHI fragment that mapped downstream from the previously characterized n/fHDK genes was cloned and sequenced. Based on nt and aa sequence similarities to n/f from other N2-fixing bacteria, eight ORF were identified and designated nifX, orf3, orfl, nifW, n/fZ, nifB, orf2 and n/fU. A region that hybridized to Rhizobium meliloti and Klebsiella pneumoniae n/fA did not appear to contain a n/fA-like gene. We have revised the map of the Fa n/f region to reflect current information.
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