Abstract
In the present study, sequences of COI gene of insects belong to five orders collected from seven cities in the Kurdistan province of Iran with other locations in the word was studied.
Highlights
In recent years the Cytochrome Oxidase I (COI) gene sequences have been used for the study of mitochondrial genes of animals
COI genes were used for differentiating and identification purposes of two near species (Helicoverpa armigera and H.assulta), so The results showed that the two species are very close in terms of phylogenetic relationships(Li, et al, 2011). when COI genes were used to the barcode of 14 species of Sweden mosquitoes, it was found that exact phylogenetic relationships between mosquito taxa were preserved at shorter evolutionary distances, but at deeper levels, they could not be inferred with confidence using COI gene sequence data alone (Engdahl et al, 2013)
We have compared between (103- 108) nucleotide sequences of Cytochrome oxidase I (COI) gene of Blatta orientalis which we have found in Kurdistan with the same nucleotide sequences of the same species living in different locations of the world (Fig. 1)
Summary
In recent years the Cytochrome Oxidase I (COI) gene sequences have been used for the study of mitochondrial genes of animals. High copy number, and lack of recombination makes many researchers choose to use the mitochondrial COI method. Some authors created three profiles of COI one for seven dominant phyla of animals, one for eight orders of insects and another for 200 closed species of Lepidoptera They have found that among the four major orders of insects, species of Diptera and Lepidoptera showed much fewer variations in their amino-acid sequences than did the Hymenoptera, while the Coleoptera showed an intermediate level of divergence (Herbert et al, 2003a) and between four orders (Coleoptera, Diptera, Lepidoptera and Hymenoptera) which were studied using Cytochrome-C oxidase divergences in the insects as a group was less than those in the other two dominant groups of arthropods, the chelicerates and crustaceans (Herbert et al, 2003b). RAPD technique was used to classify twelve species of Myrmeleontidae, and results revealed that molecular techniques could find genetic similarity between some of the species different from those already existed for long years for classification of antlions based on the use of morphological characters (Mirmoayedi et al, 2013)
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