Abstract
Thymoquinone, the main active component of the Nigella sativa seed, has been shown to have a host of potential medical applications due to its antioxidant, anti-inflammatory, hepatoprotective, and anticancer properties. Previous studies have shown that thymoquinone interacts with DNA structures such as G-quadruplex inhibiting telomerase activity. In this study, the aim was to understand the effect of DNA sequence and topology in the binding of thymoquinone to DNA. The binding of thymoquinone to phiX 174RF DNA was probed using restriction enzyme activity assays.
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