Abstract
GLI family zinc finger 3 (GLI3) has been shown to play a critical role in the regulation of Hedgehog signaling, with important implications in embryonic and postnatal development. However, the molecular characteristics of GLI3 gene and its expression profile in pig tissues have yet to be elucidated. In this study, the full-length cDNA sequence of the GLI3 gene from the lung of landrace pig was cloned and characterized using RT-PCR/RACE-PCR.
Highlights
Hedgehog (Hh) signaling has been previously demonstrated to play a critical role in embryonic patterning, organ development and tumor growth [1,2,3,4,5,6,7]
The predicted porcine GLI family zinc finger 3 (GLI3) gene (GenBank: XM_013985838.1) and 15 GLI3 sequences from different animal species were collected when the gene symbol GLI3 was queried against the GenBank database (Table 2)
The predicted porcine GLI3 gene sequence corresponding to the human GLI3 exons 10-13 was different in length from that of the other 15 GLI3 genes
Summary
Hedgehog (Hh) signaling has been previously demonstrated to play a critical role in embryonic patterning, organ development and tumor growth [1,2,3,4,5,6,7]. Hh signaling pathway is well conserved from Drosophila to human. This pathway involves several essential components, including extracellular Hh ligands (Shh, Ihh or Dhh), membrane receptors Patched (Ptc) and Smoothened (Smo), and intracellular transcription factors, GLIs [8]. GLI1, GLI2, and GLI3, function as transcription factors that regulate the Hh signaling cascade. GLIs proteins have been shown to be invloved in embryonic patterning, organ development, and homeostasis [9]. Among these three transcription factors, GLI3 has become of great interest in the field due to its bifunctional nature. In the presence of Shh signaling, full-length GLI3 functions as a transcriptional activator (GLI3A). In the absence of Shh signaling, GLI3 is partially proteolyzed into a truncated transcriptional repressor (GLI3R) to block the expression of downstream target genes [10,11]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
