Sequence Analysis of the Geranylgeranyl Pyrophosphate Synthase Gene in Cabbage

Abstract

Geranylgeranyl pyrophosphate synthase (GGPPS) is an important enzyme in carotenoid biosynthesis. Here, the Brassica oleracea var. capitata GGPPS (BocGGPPS) gene sequences were obtained from Brassica database (BRAD), and preformed for 2.1. sequence analysis. The BocGGPPS1, BocGGPPS2 and BocGGPPS3 genes mapped to chromosomes 2,3 and 9, and contains an open reading frame of 1,113 bp, 1,077 bp and 1, 116 bp that encodes a 370, 358, 371 amino acid protein, respectively. Subcellular localization predicted all BocGGPPS genes were in the chloroplast. The conserved domain of the BocGGPPS protein is Trans_IPPS_HT. Homology analysis indicates that the levels of identity among BocGGPPSs were all more than 55%, and the GGPPS protein is relatively conserved during plant evolution. The findings of the present study provide a molecular basis for the elucidation of GGPPS gene function in cabbage.