Septilin: A versatile anticlastogenic, antigenotoxic, antioxidant and histoprotective herbo-mineral formulation on cisplatin-induced toxicity in mice

Abstract

Septilin (Spt), a herbo-mineral formulation contains the extracts of Maharasanadi qoath, Tinospora cordifolia, Rubia cordifolia, Emblica officinalis, Moringa pterigosperma, Glycyrrhiza glabra and powders of Balsamodendron mukul and Shankha bhasma. In the present study, the anticlastogenic, antigenotoxic, antioxidant and histoprotective effects of Spt against cisplatin (Csp) induced toxicity in Swiss albino mice were investigated. The micronucleus (MN) test was used to assess the anticlastogenic potential of Spt (125, 250 and 500 mg/kg bw; p.o., 5 days) on somatic cells of mice. The sperm shape abnormality assay detects germinal nuclear damage, which induces spermatogenic dysfunction. Comet assay was employed to study the antigenotoxic potential of Spt on Csp (10 mg/kg bw; i.p.) induced DNA strand breaks in bone marrow cells of mice. The antioxidant enzyme activity of liver superoxide dismutase (SOD) and a biological antioxidant, reduced glutathione (GSH) were measured to determine its hepatoprotective property. The ability of Spt to protect against the histopathologic alterations accompanying Csp-induced liver and testicular injury was studied. The frequencies of MN induced by Csp in bone marrow and peripheral blood cells of mice were significantly decreased by the pre-treatment of Spt. Csp treatment increased the percentage of DNA strand breaks and depleted levels of SOD and GSH content along with histopathological changes. Supplementation of Spt attenuated the toxicity of Csp in liver and testes tissues possible viaimprovement of enzymatic and histological parameters toward normal. This study suggests that the protection offered by Spt against Csp-induced toxicity is partly related to the maintenance of the antioxidant system. Overall, this study shows the protective role of Spt against Csp-induced toxicity in somatic and male germinal cells of mice.