Abstract
Abstract Methods are presented for the separation of the phosphate esters of thiamin, oxythiamin or pyrithiamin and the separation of thiamin and oxythiamin by high-performance liquid chromatography. Detection by means of a UV monitor and fluorometer with a post-column oxidation system is described which allows for selective detection of thiochrome, pyrichrome and their respective phosphate esters. Anion exchange and ion-pair reverse phase chromatography are the chromatographic modes of separation.
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