Abstract
ABSTRACTLipoprotein and water‐soluble proteins were separated from egg yolk with sodium alginate. Ninety‐nine percent of the lipids were precipitated and 20% of protein remained in the supernatant by this treatment. SDS‐PAGE showed the presence of α‐, β‐, ‐γ‐livetins in the supernatant. Phospholipids were purified from the precipitated lipoproteins by 95% ethanol extraction, then ZnCl2 precipitation, and subsequent acetone washing. Phospholipids recovery and purity were 78.5% and 95.9%, respectively. Phospholipids thus purified gave a clear colorless solution in chloroform and were free of triglycerides and cholesterol on TLC plates.
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