Abstract
Size-exclusion chromatography is employed for the separation of gold nanoparticles in the range from 5.3 to 38.3 nm with a polymer-based column of 100 nm pore size. The sorption problem of gold nanoparticles by high surface of stationary phase can be solved with the addition of anionic surfactant to the eluent. Excellent linearity from the plot of logarithm of the particle size as a function of elution time is obtained. Also, the reproducibility of separation for the entire range of calibration curve is high. The size resolution ( R s=1.0) of 10 nm, without considering particles size distribution, is obtained from optimal conditions.
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