Separation of N-Containing Species by Means of a New Versatile Ion Interaction RP-HPLC Methodology

Abstract

Abstract A new reverse phase HPLC methodology is presented for the separation of nitrites, nitrates, aliphatic and aromatic amines contained in the same mixture. The same basic methodology can be made very versatile by properly varying the experimental chromatographic conditions. Fundamentally, the proposed technique makes use of reverse phase RP-18 columns and of two interaction reagents, namely octylaminium salicylate and octylaminium ortho-phosphate. Conductometric and spectrophotometric (both direct and indirect) detections are employed. Through the appropriate choice of mobile phase, kind of detection and detection wavelength, the methodology makes possible the separation and the definite identification of N-containing species, both inorganic and organic. The method can be very usefully employed in analysis both of drinking water and of waste water.