Abstract
The effects of pH, ionic strength and amount of methanol in the eluent on the retention of 5′-, 3′- and 2′-ribonucleoside monophosphates on a reversed-phase high-performance liquid chromatographic system are described. The data were used to develop suitable separation protocols for synthetic nucleotide mixtures and applied to the separation of RNA nucleotides derived by alkaline hydrolysis.
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Schedule a callMore From: Journal of Chromatography B: Biomedical Sciences and Applications
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