Abstract
A chromosome sorting technique is described based on velocity sedimentation at unit gravity which combines simplicity with good resolution. Large quantities of chromosomes (up to 10 10) can be sorted for further biological and biochemical research. Male Indian muntjac cells were first synchronized and subsequently accumulated in mitosis with vinblastine. After hypotonic treatment, mitotic cells were disrupted and the crude chromosomal preparation was layered as a thin film onto a two-step linear sucrose gradient in a specially designed sedimentation chamber (area 127.6 cm 2). After 18 h of sedimentation at unit gravity, fractions were collected and the chromosomal composition of the fractions was analyzed by flow cytometry and microscopy.
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