Abstract
A method for isolation of mast cells from murine peritoneal cell suspensions is described. Mast cells are isolated by velocity sedimentation at unit gravity on a linear gradient (generated in a discontinuous step fashion) of colloidal silica particles coated with polyvinyl pyrrolidone (Percoll) in Fischer's medium supplemented with 2% fetal bovine serum. The overall recovery of the peritoneal cells layered on the gradient is 90–95%, while the yield of mast cells is 77% with an average purity of ⩾95%.
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