Abstract
The separation of glucosamine, galactosamine, muramic acid and the corresponding aminodeoxyalditols as their 2,4-dinitrophenyl (DNP) derivatives by two- dimensional thin-layer chromatography on polyamide is described. Reaction conditions are recommendation that minimize artefact formation during derivatisation. Initial development with 2% (v/v) acqueous formic acid separates the DNP derivatives from salts and by-products, which otherwise interfere with separation. Solvents for the second dimension contain chlorobenzene and acetic acid, with ethylenediamine- tetra-acetic acid to reduce tailing. Inclusion of benzeneboronic acid is particularly effective for separating compounds of interest.
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