Abstract
Different packed capillary columns were evaluated for carotenoid separation by subcritical fluid chromatography with neat CO 2. Packed capillary columns studied involved the use of silica particles deactivated with different methods and particles coated with stationary phases commonly used in gas chromatography, such as SE-54 (95% methyl-, 5% phenylsilicone) and OV-17 (50% methyl-, 50% phenylsilicone). In order to select the appropriate chromatographic conditions to elute carotenoids, a theoretical study of the solubility of β-carotene was performed. Separation of lycopene and β-carotene was obtained with neat CO 2 working at pressures around 300 atm and at a temperature of 10°C (1 atm=101 325 Pa). Evaluation of the columns used in the present study in terms of efficiency and activity was also performed.
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