Abstract
Liquid and subcritical chromatographic analyses using evaporative light scattering detection were used to investigate the composition of various commercial dimethylated β-cyclodextrins (β-CD). The chromatographic fingerprints better depict the complexity of each mixture than the degree of substitution. The elution order is reversed when subcritical fluid chromatography is used instead of LC on apolar stationary phases (TMS and phenyl columns). Concerning the RPLC analysis of methylated β-CD, a phenyl-bonded silica column offers selectivities that are different from those obtained using a classical C 18-bonded silica column. With regard to the more complex mixtures, SubFC allows one to obtain richer methylated β-CD fingerprints than does LC.
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