Abstract
A method for the separation of biological cells and bacteria based on traveling wave dielectrophoresis (twDEP) is presented. The microfluidic chip consists of a structured PDMS layer on glass. Parallel electrodes with increasing width and gap size, positioned along the micro fluidic channel, were used to expose cells to a twDEP force perpendicular to a pressure driven flow. With this gradient electrode structure we show that successful separations of mixtures of bacteria contaminated cells into subpopulations of viable cells and bacteria are feasible.
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