Abstract
ALTHOUGH cellulose column chromatography1 is generally useful for the separation of anthocyanin mixtures, difficulties may be encountered in applying this technique in the presence of large amounts of other flavonoid materials. Thus, in the case of complex petal extracts, overlapping of bands can occur so that the anthocyanins are contaminated with other pigments; and in the case of extracts containing mobile leuco-anthocyanins, the anthocyanin bands are located within a broad diffuse zone containing the former compounds. While these difficulties can be overcome by further purification involving partition between solvent systems, or repeated cellulose column chromatography, a very convenient and effective method is provided by preliminary treatment on a column of polyamide2–4.
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