Abstract
The immune system consists of a complex array of specialized and interacting cells, a fact that challenges, delights, and confuses the immunologist. A direct experimental approach to this situation would be to separate out and classify individual types of cells so that their immunological responses and the nature of their interactions could be studied under controlled experimental conditions. The technology for sorting out cells from the dauntingly complex mixtures in lymphoid organs is a recent development, and “biological” separation of cells, or the use of cell-specific antisera, has been responsible for the major advances so far. For example, the use of bursectomized or thymectomized animals, and the use of antilymphocyte antisera has led to the functional distinction between B and T lymphocytes.3 However, the complexity of lymphocyte populations extends beyond this primary division, and it is evident that in future, separation procedures will be of major importance.
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