Abstract
As a pretreatment technique to trace analysis, polyoxyethylene lauryl ether (POELE10)- salt aqueous two-phase extraction system (ATPES) is coupled with high performance liquid chromatography to analyse thiamphenicol (TAP) in egg, milk and honey. The analysis of the phase behaviors of the ATPES found that the extraction efficiency and enrichment factor of TAP was influenced by the types of salts, the concentration of salt, the concentration of POELE10, the concentration of 1-propanol and the temperature. The optimized conditions of experiment were determined in the multi-factor experiment by using response surface methodology. The extraction efficiency of TAP is up to 99.59% and the optimal values of enrichment factor of TAP was 27 under the optimized conditions. The limit of detection and the limit of quantification of TAP are 0.5 µg kg−1 and 1.5 µg kg−1, which meet the needs of determination of trace or ultratrace TAP in food.
Highlights
Thiamphenicol1 [D(+)-threo-2-di-chloroacetamido-1(4-methylsulphonylphenyl) propane-1, 3-diol; (TAP)] is an analogue of chloramphenicol in which the nitro group on the benzene ring is replaced with a methylsulfonic group (Figure 1).[2]
We studied the extraction and determination of TAP by using POELE10-salt Aqueous two-phase system (ATPS) coupled with high performance liquid chromatography (HPLC)
The extraction efficiency and enrichment factor of the various ATPSs for TAP at three temperatures are shown in Figures 3 and 4
Summary
Thiamphenicol1 [D(+)-threo-2-di-chloroacetamido-1(4-methylsulphonylphenyl) propane-1, 3-diol; (TAP)] is an analogue of chloramphenicol in which the nitro group on the benzene ring is replaced with a methylsulfonic group (Figure 1).[2].
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