Separation, concentration and determination of trace chloramphenicol in shrimp from different waters by using polyoxyethylene lauryl ether-salt aqueous two-phase system coupled with high-performance liquid chromatography

Abstract

Polyoxyethylene lauryl ether (POELE10)-NaH2PO4 aqueous two-phase extraction system (ATPES) is coupled with HPLC to analyze chloramphenicol (CAP) in aquatic product. Response surface methodology (RSM) was adopted in the multi-factor experiment to determine the optimized conditions. The extraction efficiency of CAP (E%) is up to 99.42% under the optimal conditions, namely, the concentration of NaH2PO4, the concentration of POELE10, pH and temperature were 0.186g·mL−1, 0.033g·mL−1, 3.8 and 25°C respectively. The optimal value of enrichment factor of CAP (F) was 22.56 when the concentration of NaH2PO4 was 0.192g·mL−1, the concentration of POELE10 was 0.024g/ml, pH was 4.2 and temperature was 30°C. The limit of detection (LOD) and limit of quantification (LOQ) of this method are 0.8μg·kg−1 and 1μg·kg−1, which meet the needs of determining trace or ultratrace CAP in food. The E% and F of this technique are much better than other extraction methods.