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Abstract
The separation behavior of red blood cells (RBCs) in sedimentation field-flow fractionation (SdFFF) was investigated under higher field and flow conditions than used previously. This work shows that SdFFF separation of RBCs is fast with the total operating time less than 5 minutes. The resolution is high and the cells retain their integrity after separation. The optimization of cell separation by SdFFF was investigated. Differences in retention ratios of RBCs from various mammals shows that red cells are separated in the lift-hyperlayer mode based mainly on size differences. Lift forces, especially those originating from deformability and inertial effects play important roles in the separation of red cells and other deformable particles.
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