Abstract
Glycyrrhizin (GL) is a triterpenoid saponin that is commonly isolated from root of Glycyrrhiza glabra L. an indigenous medicinal and culinary herb. The present study focused on detecting and optimizing isolation method to isolate GL from successive root extracts of G. glabra L. in its purest form with high yield. The study exploited cold percolation extraction and variety of chromatographic methods such as HPTLC, HPLC and gradient column chromatography. The comparative HPTLC densitometry identified GL as a single grey color band at RF 0.36 under UV-254nm in successive extracts. The optimized extraction-isolation method isolated the in-house GL from 70% aqueous ethanol extracts using standardized eluent, i.e., ethyl acetate-methanol (7:3, v/v) and confirmed by Co-TLC. The compound obtained as colorless non sticky solid, with a yield 0.4%. Various spectroscopic tools characterized isolated GL along with HPLC peak detection, this confirmed its identity by matching with reported literature. The proposed extraction–isolation method could be utilized to enhance the efficacy of herbal pharmaceuticals, development of advanced Ayurvedic formulations and uplifting the quality of food products, and cosmeceuticals contain GL as active constituents. Additionally, this work established a simple, fast, and cost-effective HPTLC method that may also be applied to detect GL to establish genuineness of such raw drugs, identification of Ayurvedic dosages, and studying such triterpenoid saponins in other medicinal species belonging Glycyrrhiza for routine quality control.
Published Version
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