Abstract
Quercetin is a polyphenolic flavonoid found in many plants and is used as a nutritional supplement. It has been reported to have biological properties that may play an important role in the prevention of human diseases, such as cancer, cardiovascular diseases, diabetes, ulcer, cataract and allergies. In this research, solidified floating organic drop microextraction coupled to high-performance liquid chromatography was used to extract and determine quercetin in Rosa canina L., growing in different areas of Lorestan Province in Iran. The effect of important experimental variables such as type and volume of extracting organic solvent, extraction temperature and time, pH and effect of ionic strength on the extraction recovery was investigated and optimized. Under the optimum extraction conditions, a linear response was obtained over the range of 0.5–40 µg mL−1. Relative standard deviation (RSD%) and enrichment factor were found to be 3.4% and 230, respectively. The proposed method was applied successfully for the determination of quercetin in calycles and fruit of R. canina L. samples. The results showed good agreement compared with those obtained by a validated standard method .
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.