Abstract
Procedures are described for the separation and quantitative estimation of betaines as their p-bromophenacyl, phenacyl, p-nitrobenzyl or methyl esters. The aromatic esters are separated on weak or strong cation-exchange columns eluted with a choline buffer in 5–30% aqueous acetonitrile and detected with a variable-wave-length UV absorbance detector. A Dionex ion chromatography is used for the separation of methyl esters on a neutral column in the presence of hexanesulphonic acid. In this case a conductivity detector is used after suppression of the background conductivity of the eluant. Both methods offer improved sensitivity and reduced background noise when compared with the direct separation of betaines and their detection by UV absorbance at 200 nm.
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