Abstract
The simultaneous separation of a mixture of equine estrogens containing estrone, equilin, equilenin and their corresponding 17α-diols was accomplished by reversed-phase high-performance liquid chromatography (RP-HPLC) on a Nucleosil C 18 column with a mobile phase composed of methanol-water-2-propanol-dichloromethane (45:42.5:7.5:5, v/v). UV absorbance and electrochemical detection were used for the analysis of a standard mixture of equine estrogens. RP-HPLC with UV absorbance detection was applied to the determination of these compounds in pharmaceutical drug substances and drug products after acid hydrolysis of their sodium sulfate esters.
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