Abstract
Several elution systems for the separation and the isolation of underivatized and pyridylethylated highmolecular-weight (HMW) subunits of glutenin by RP-HPLC on Nucleosil C8 are presented. A 2-propanol/trifluoroacetic acid/acetonitrile system proved successful for micropreparative purposes, while an urea-containing system with an extremely flat gradient delivered the best quantitative results, because of its high resolving power. The HMW subunits of 24 wheat varieties with different functional properties were quantitatively analyzed. The values obtained were correlated with physical parameters, namely SDS-sedimentation volume and maximum resistance. The results clearly demonstrated the significance of the type, as well as the amount, of HMW sub-units relative to the functional properties.
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